{"id":12347,"date":"2023-10-23T09:26:08","date_gmt":"2023-10-23T09:26:08","guid":{"rendered":"https:\/\/ibima.eu\/?page_id=12347"},"modified":"2023-10-26T06:32:42","modified_gmt":"2023-10-26T06:32:42","slug":"electron-microscopy","status":"publish","type":"page","link":"https:\/\/ibima.eu\/en\/electron-microscopy\/","title":{"rendered":"ELECTRON MICROSCOPY"},"content":{"rendered":"<p>[et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/04\/BANNER_2.jpg\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_2_font=\u00bbPoppins|500|||||||\u00bb header_2_text_color=\u00bb#FFFFFF\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h2>ELECTRON MICROSCOPY<\/h2>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb hover_enabled=\u00bb0&#8243; global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0&#8243;][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb hover_enabled=\u00bb0&#8243; border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0&#8243;][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Service Description<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>The Electron Microscopy Service offers access to transmission electron microscopy (TEM), cryo-TEM and electron tomography, high resolution scanning electron microscopy (SEM), and environmental SEM. A special emphasis has been made on high-end sample preparation techniques through cryo-immobilization. All the equipment has been configured to provide its best for biological and nanomedicine applications.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb hover_enabled=\u00bb0&#8243; global_colors_info=\u00bb{}\u00bb border_width_left=\u00bb1px\u00bb border_color_left=\u00bb#41a7d4&#8243; sticky_enabled=\u00bb0&#8243; custom_padding=\u00bb|||50px|false|false\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]Equipment[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb hover_enabled=\u00bb0&#8243; global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0&#8243;]<\/p>\n<p><strong>&#8211; Thermo ScientificFEI Tecnai G2 20 Twin Transmission Electron Microscope<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">LaB<\/span><span style=\"font-weight: 400;\">6<\/span><span style=\"font-weight: 400;\"> filament\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Tomography software<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Cryo-transmission system\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">EDS\/ EDX (Energy Dispersive X-ray Spectroscopy) elemental analyzer\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>&#8211; Thermo ScientificFEI Quanta 250 FEG Scanning Electron Microscope<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Field emission gun\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Environmental-SEM\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Wet-STEM\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">EDS\/ EDX (Energy Dispersive X-ray Spectroscopy) elemental analyzer\u00a0<\/span><\/li>\n<\/ul>\n<p><strong>&#8211; Sample preparation equipment<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Thermo Scientific-FEI Vitrobot Mark IV, for plunge-freezing of water-solution samples\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Gatan Turbo Pumping Station, for conditioning of the cryo-holder under vacuum conditions<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">GatanCryo Transfer Workstation, for transferring of grids to the cryo-holder under liquid nitrogen-temperature conditions\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">GatanSmartSet Model 900 Cold Stage Controller, digital controller for temperature conditioning of the cryo-holder\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Leica ultramicrotome UC7, with cryo-ultramicrotomy module FC7, for semithin and ultrathin sections\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Leica HPM100, for high pressure freezing cryo-fixation of samples at low temperatures<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">\u00a0Leica AFS2, for freeze substitution resin-embedding of samples at low temperatures, with automatic reagent dispenser FSP<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Leica SCD500, for metal-thin film deposition on samples for scanning electron microscopy, and glow-discharge of film-coated grids for transmission electron microscopy<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Leica KMR3, glass-knife maker for ultramicrotomy<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Leica MP, mounting hot-plate for plastic boats on glass knives<\/span><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_divider color=\u00bb#41a7d4&#8243; divider_style=\u00bbdotted\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]Applications[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>&#8211; Cellular characterization: cell structures and ultrastructures visualization and its relationship to function are the most important contributions of EM to cell biology &#8211; &#8211; This includes not only standard visualization of embedded and stained sections but also localization of proteins inside cells through immunolabelling with a resolution around 10 nm For this high-resolution analysis, the application of sample preparation methods that preserve the ultrastructure is essential High-pressure freezing is the cryo-immobilization technique preferred for this kind of characterization Moreover, electron tomography is uniquely suited to obtain 3D reconstructions of cells and their internal structures and, thus, improve the information provided by standard 2D analysis<\/p>\n<p>&#8211; Tissue characterization: electron microscopy has a role in the characterization of interactions between cells and with other components inside tissues Moreover, high-resolution surface characterizations of bones, teeth, skin, etc, are typically performed by SEM<\/p>\n<p>&#8211; Other biological systems: leaves, flowers, insects, and microorganisms are among the structures that can be topographically characterized using SEM For these systems, as for others with high water content, ESEM has become an important tool due to its ability for surface visualization even in a 100% humidity environment, reducing sample preparation and permitting dynamic analysis through hydration-dehydration cycles Dynamics experiments are useful in Materials Science, food industry, water-oil emulsions characterization, and pharmaceutical quality control<\/p>\n<p>&#8211; Biomaterials characterization: electron microscopy plays a double role in biomaterial characterization On one hand, it provides structural and compositional information on the materials engineered to be used inside biological systems, favoring its development On the other hand, it allows us to visualize their interactions Functionalized polymeric and metallic nanoparticles for drug delivery, dental implants, bone plates and cements, and artificial tissues are among the biomaterials that can benefit from the information provided by EM<\/p>\n<p>&#8211; Macromolecular complexes characterization: structural biology can benefit from electron microscopy to determine 3D structures of macromolecular complexes &#8211; &#8212; &#8211; &#8211;<\/p>\n<p>&#8211; Negative staining was typically employed in the past, but it has been the development of cryo-electron microscopy in combination with single particle analysis that has made EM such a very valuable tool for 3D characterization<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb] Services Offered[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>&#8211; Transmission electron microscopy of resin-embedded and non-embedded samples and elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX\/ EDS).<\/p>\n<p>&#8211; Electron Tomography of room temperature (conventional) and frozen-hydrated samples (cryo-electron tomography)<\/p>\n<p>Scanning electron microscopy for structural studies of samples and elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX or EDS).<\/p>\n<p>&#8211; Environmental scanning electron microscopy of hydrated samples and dynamic experiments.<\/p>\n<p>&#8211; Wet-scanning transmission electron microscopy of samples in thin-film water solution.<\/p>\n<p>&#8211; Resin embedding of samples at room temperature (conventional) or low temperature (freeze-substitution) for ultramicrotomy.<\/p>\n<p>&#8211; Plunge-freezing of samples in water solution (vitrification) for cryo-transmission electron microscopy.<\/p>\n<p>&#8211; Cryo-fixation of samples at high pressure (High-Pressure Freezing).<\/p>\n<p>&#8211; Ultramicrotomy of resin-embedded sections (semithin and ultrathin sections).<\/p>\n<p>&#8211; Glow-discharge of film-coated transmission electron microscopy grids.<\/p>\n<p>&#8211; Data and image processing.<\/p>\n<p>&#8211; Technical advice for users regarding the selection of protocols and procedures for experiments involving electron microscopy.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_color=\u00bb#235b78&#8243; custom_padding=\u00bb30px||30px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_3_font=\u00bbPoppins|700|||||||\u00bb header_3_text_color=\u00bb#FFFFFF\u00bb header_3_font_size=\u00bb34px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h3>Technical staff<\/h3>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb50px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_blurb title=\u00bbL\u00d3PEZ T\u00c9LLEZ, JUAN F\u00c9LIX\u00bb image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/10\/NANJFT.jpeg\u00bb image_icon_width=\u00bb40%\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_font=\u00bbPoppins|600|||||||\u00bb header_text_align=\u00bbcenter\u00bb body_text_align=\u00bbcenter\u00bb border_radii_image=\u00bbon|80px|80px|80px|80px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Laboratory technician<br \/>952 367 627<br \/>juanfelix.lopez@ibima.eu<\/p>\n<p>[\/et_pb_blurb][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb||50px||false|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_blurb title=\u00bbOCA GIL, CONCEPCI\u00d3N\u00bb image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/10\/NANCOG.jpeg\u00bb image_icon_width=\u00bb40%\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_font=\u00bbPoppins|600|||||||\u00bb header_text_align=\u00bbcenter\u00bb body_text_align=\u00bbcenter\u00bb border_radii_image=\u00bbon|80px|80px|80px|80px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Unit Technician<br \/>Technicianfor the Unit<br \/>cocal@ibima.eu; cultivosycitometria@ibima.eu<\/p>\n<p>[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"<p>ELECTRON MICROSCOPYService DescriptionThe Electron Microscopy Service offers access to transmission electron microscopy (TEM), cryo-TEM and electron tomography, high resolution scanning electron microscopy (SEM), and environmental SEM. A special emphasis has been made on high-end sample preparation techniques through cryo-immobilization. All the equipment has been configured to provide its best for biological and nanomedicine applications.Equipment- Thermo [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>ELECTRON MICROSCOPY - Ibima<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibima.eu\/en\/electron-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ELECTRON MICROSCOPY - Ibima\" \/>\n<meta property=\"og:description\" content=\"ELECTRON MICROSCOPYService DescriptionThe Electron Microscopy Service offers access to transmission electron microscopy (TEM), cryo-TEM and electron tomography, high resolution scanning electron microscopy (SEM), and environmental SEM. 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