[et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb background_image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/04\/BANNER_2.jpg\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb header_2_font=\u00bbPoppins|500|||||||\u00bb header_2_text_color=\u00bb#FFFFFF\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2″ use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4″ global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4″ text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n
Service Description<\/p>\n
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Laser-scanning confocal microscopy is a very popular technique that uses a combination of laser illumination and a \u201cpinhole\u201d mask to ensure that only fluorescence from the focal plane reaches the detector. This avoids the characteristic blur typical of conventional fluorescence microscopy and allows images to captured as detailed optical slices that can be used to build up rich 3D models. Confocal microscopy is one of the most versatile and widely used techniques in optical microscopy. We offer Leica SP5 confocal microscopes featuring HyD hybrid detectors for the best possible sensitivity, as well as full environmental control for live cells and high-speed resonant scanning.<\/p>\n
Multi- or two-photon (2P) microscopy takes advantages of the near simultaneous absorption of two or more photons which act to excite a fluorescent molecule with the combined energy of all the photons. In practice, this means that lower energy infrared (IR) light can be used to see fluorescent molecules that are normally excited by high energy ultraviolet and visible wavelength. As IR light is less susceptible to diffusion and absorption, we can visualise fluorescent molecules at greater depths than conventional microscopy. Infrared light also tends to beless damaging to live tissues than UV or blue excitation, making it ideal for timelapse imaging of model organisms or tissue explants.<\/p>\n
[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4″ text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]Equipment[\/et_pb_text][et_pb_text _builder_version=\u00bb4.24.2″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb hover_enabled=\u00bb0″ global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0″]<\/p>\n
– ZEISS LSM 980 confocal optical microscopy unit with multiphoton module (NLO). Co-financed Infrastructure by NextGeneration EU – Recovery and Resilience Mechanism: ICT2022-007849 \u201cActualizaci\u00f3n de la infraestructura del nodo Bionand de Nanbiosis\u201d.<\/p>\n
– Leica SP5 HyD Confocal Microscope<\/p>\n
– Leica SP5 HyD MP Multiphoton\/Confocal Microscope<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_divider color=\u00bb#41a7d4″ divider_style=\u00bbdotted\u00bb _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_2,1_2″ use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4″ global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4″ text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]Applications[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]- 3D imaging of fixed multi-color immunofluorescence preparations
\nLong-term and high-speed live cell imaging<\/p>\n
– FRAP (Fluorescence Recovery after Photobleaching) and photoactivation methods for studying molecular dynamics<\/p>\n
– Foster Resonance Energy Transfer (FRET) for studying molecular interactions at sub\u2013nanometric distances<\/p>\n
– Characterization of single (1P) and two (2P) photon fluorescence properties of novel materials in vitro and in vivo<\/p>\n
– Two-photon deep tissue imaging (>100 microns) of fluorescent proteins[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4″ text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n
Services Offered<\/p>\n
[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]- Experimental design advice and consulting for project planning<\/p>\n
– Integrated cellular interaction analyses; we offer rapid assays analyzing uptake and subcellular localization of fluorescently-labeled molecules in standard cultured cell lines<\/p>\n
– Quantitative, semi-quantitative, and comparative analyses of fluorescent expression\/staining in different models. We can advise on the required controls or limitations of different methodologies<\/p>\n
– Co-localisation studies comparing localization with standard sub-cellular markers, fluorescent proteins, and antibodies with rigorous statistical analysis performed using commercial (IMARIS) and open-source (FIJI) co-localization analysis tools<\/p>\n
– We specialize in the long-term (>4d) microscopic visualization of cell models, including primary cells, model organisms, and bacterial colonies<\/p>\n
– Sample preparation services include fixation, antibody staining, and sample mounting. Where required, we can troubleshoot and optimize difficult staining combinations<\/p>\n
– We can supply small aliquots of a wide range of standard staining reagents, fluorophores, and secondary antibodies, and we also offer a custom antibody labeling service<\/p>\n
– 3D quantification and visualization using the advanced IMARIS 3D analysis package, including volume quantification, 3D object tracking, and cell type and subcellular organelle counting<\/p>\n
– Two-photon emission and excitation spectra measurements and Quantum efficiency (QE) estimation by comparison with reference compounds<\/p>\n
– High-resolution intravital imaging of sub-surface tissues taking advantage of higher tissue penetration of two-excitation and the long working distance 25x water immersion objective[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb background_color=\u00bb#235b78″ custom_padding=\u00bb30px||30px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb header_3_font=\u00bbPoppins|700|||||||\u00bb header_3_text_color=\u00bb#FFFFFF\u00bb header_3_font_size=\u00bb34px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb50px||50px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_blurb title=\u00bbMOLINA GIL, SARA\u00bb image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/05\/person-icon-1.png\u00bb image_icon_width=\u00bb40%\u00bb _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb header_font=\u00bbPoppins|600|||||||\u00bb header_text_align=\u00bbcenter\u00bb body_text_align=\u00bbcenter\u00bb border_radii_image=\u00bbon|80px|80px|80px|80px\u00bb global_colors_info=\u00bb{}\u00bb]T\u00e9cnico de Microscop\u00eda
\nMicroscopy Technician[\/et_pb_blurb][\/et_pb_column][et_pb_column type=\u00bb1_2″ _builder_version=\u00bb4.20.4″ _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb||50px||false|false\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"
Confocal and Multiphoton MicroscopyService DescriptionLaser-scanning confocal microscopy is a very popular technique that uses a combination of laser illumination and a \u201cpinhole\u201d mask to ensure that only fluorescence from the focal plane reaches the detector. This avoids the characteristic blur typical of conventional fluorescence microscopy and allows images to captured as detailed optical slices that […]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"acf":[],"yoast_head":"\n