{"id":12386,"date":"2023-10-25T07:02:39","date_gmt":"2023-10-25T07:02:39","guid":{"rendered":"https:\/\/ibima.eu\/?page_id=12386"},"modified":"2023-10-26T06:59:17","modified_gmt":"2023-10-26T06:59:17","slug":"high-content-screening","status":"publish","type":"page","link":"https:\/\/ibima.eu\/en\/high-content-screening\/","title":{"rendered":"HIGH CONTENT SCREENING"},"content":{"rendered":"<p>[et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/04\/BANNER_2.jpg\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_2_font=\u00bbPoppins|500|||||||\u00bb header_2_text_color=\u00bb#FFFFFF\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h2>HIGH CONTENT SCREENING<\/h2>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Service Description<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]High content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. HCS combines automated microscopy with customizable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells. The Perkin Elmer Operetta HCS system combines automated imaging with advanced Harmony HCS software, which contains many common algorithms such as nuclei and cell detection, as well as the ability to automatically detect complex phenotypes through machine learning.[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb hover_enabled=\u00bb0&#8243; global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0&#8243;]<\/p>\n<p>Equipment<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb hover_enabled=\u00bb0&#8243; global_colors_info=\u00bb{}\u00bb sticky_enabled=\u00bb0&#8243;]<\/p>\n<p>&#8211; Perkin Elmer Operetta HCS<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">10x, 20x and 40x objective lenses.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Temperature control and CO<\/span><span style=\"font-weight: 400;\">2<\/span><span style=\"font-weight: 400;\"> for cultured cells.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Fully automated image acquisition from 24,96 and 384 well plates.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Latest version Harmony 4.6: integrated acquisition and analysis software.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Real-time automated analysis and quantification of image data.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Machine learning-based phenotype recognition algorithms (Phenologic)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Offline Harmony analysis workstation for improved throughput.<\/span><\/li>\n<\/ul>\n<p>&#8211; Thermofisher Versette liquid handling robot for 96-well plates<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Programmable robot system<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">6 position stage: exchange between up to 6 plates or reservoirs\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Suitable for plate cloning and serial dilutions<\/span><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_divider color=\u00bb#41a7d4&#8243; divider_style=\u00bbdotted\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Applications<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]- Fast and statistically robust toxicity assays using adherent cells with the advantage of being able to visually confirm cell morphology<\/p>\n<p>&#8211; Drug dose-response curve calculation: On a single plate, multiple concentrations or combinations of compounds can be easily and quickly tested<\/p>\n<p>&#8211; Screening for specific phenotypes in cultured cells using libraries of small molecules, siRNAs, or other biologically-active collections<\/p>\n<p>&#8211; Screening for complex phenotypes: many cytological effects cannot be detected easily by cytometry Operetta allows complex phenotypes (eg organelle distribution or cell organization changes) to be detected automatically and reliably<\/p>\n<p>&#8211; Long-term cell culture studies: Operetta is equipped with temperature and CO2 control, making it suitable for maintaining live cultured cells in optimal conditions during image acquisition[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb] Services Offered[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<ul>\n<li>High content screening design and setup advice The most important step in performing a successful HCS screen is good experimental design and planning<\/li>\n<li>Experimental validation and pilot study testing For more complex projects, it is essential to perform preliminary tests to identify possible problems with labeling, marker expression, cell morphology, and other sources of variability before expending valuable time and reagents<\/li>\n<li>Custom experiments and HCS analysis design: we can design both assays and custom automatic image analysis pipelines using the Harmony platform based on your requirements<\/li>\n<li>Integrated cytotoxicity testing service We have developed robust protocols from the incubation of cultured cells with test compounds to measuring cytotoxic responses at different time points Different criteria such as plasma membrane integrity, metabolic activity, or intracellular esterase activity can be chosen depending on experimental requirements<\/li>\n<li>Development of custom liquid handing protocols Using the Thermofisher Versette liquid handing system, we can develop tailor-made solutions to facilitate medium-to-large scale experiments in 96 well plates with greater accuracy and lower overall costs<\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_color=\u00bb#235b78&#8243; custom_padding=\u00bb30px||30px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_3_font=\u00bbPoppins|700|||||||\u00bb header_3_text_color=\u00bb#FFFFFF\u00bb header_3_font_size=\u00bb34px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h3>Technical staff<\/h3>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb50px||50px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_blurb title=\u00bbMOLINA GIL, SARA\u00bb image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/05\/person-icon-1.png\u00bb image_icon_width=\u00bb40%\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_font=\u00bbPoppins|600|||||||\u00bb header_text_align=\u00bbcenter\u00bb body_text_align=\u00bbcenter\u00bb border_radii_image=\u00bbon|80px|80px|80px|80px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Microscopy Technician<br \/>Microscopy Technician<\/p>\n<p>[\/et_pb_blurb][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb||50px||false|false\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>","protected":false},"excerpt":{"rendered":"<p>HIGH CONTENT SCREENINGService DescriptionHigh content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. HCS combines automated microscopy with customizable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells. The Perkin Elmer [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>HIGH CONTENT SCREENING - Ibima<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibima.eu\/en\/high-content-screening\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HIGH CONTENT SCREENING - Ibima\" \/>\n<meta property=\"og:description\" content=\"HIGH CONTENT SCREENINGService DescriptionHigh content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. 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