{"id":12461,"date":"2023-10-26T06:32:57","date_gmt":"2023-10-26T06:32:57","guid":{"rendered":"https:\/\/ibima.eu\/?page_id=12461"},"modified":"2024-07-25T15:28:47","modified_gmt":"2024-07-25T15:28:47","slug":"super-resolution-and-conventional-fluorescence-microscopy","status":"publish","type":"page","link":"https:\/\/ibima.eu\/es\/super-resolution-and-conventional-fluorescence-microscopy\/","title":{"rendered":"Super-resolution and Conventional Fluorescence Microscopy"},"content":{"rendered":"<p>[et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2023\/04\/BANNER_2.jpg\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_2_font=\u00bbPoppins|500|||||||\u00bb header_2_text_color=\u00bb#FFFFFF\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h2>Super-resolution and Conventional Fluorescence Microscopy<\/h2>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Service Description<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]Conventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional micros-copy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, it also allows for faster imaging in many cases. Examples where conventional microscopy may be advantageous include the visualization of individual molecules, receptors, or small organisms such as bacteria and yeast.<br \/>\nTotal Internal Reflection Fluorescence (or TIRF) is a powerful technique that combines the sensitivity of conventional fluorescence with selective illumination to improve the contrast of features very close to the sample coverslip.   TIRF is often used for studies related to membrane dynamics, receptor-ligand interactions, and vesicular transport.<br \/>\nThe intrinsic diffraction of light has historically made it difficult to use fluorescence to distinguish structures closer than 200nm apart. Super-resolution microscopy refers to techniques that selectively activate fluorescent molecules to map their position with up to 10 times more accuracy than conventional fluorescent microscopy. The Nikon N-Storm system is capable of localizing molecules with a resolution of up to 20nm and is compatible with all most current localization protocols and fluorophores (including Alexafluor 647 and Atto488).[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|||50px|false|false\u00bb border_width_left=\u00bb1px\u00bb border_color_left=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]Equipment[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<ul>\n<li aria-level=\"1\"><b>Nikon Eclipse TiTIRF \/ N-STORM <\/b><b>(desplegable)<\/b><\/li>\n<\/ul>\n<ul>\n<ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Andor iXon3 897 EM-CCD camera.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">100x Plan Apo Oil immersion Objective 1.49 NA.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">4 Excitation lasers405nm, 488nm (200mW), 561nm and 647nm (300mW)\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">TIRF optical system.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Multipass TIRF and STORM filters for high-speed multi-color imaging.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Removable cylindrical lens for 3D STORM.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Nikon PFS (Perfect Focus System) for improved stability<\/span><\/li>\n<\/ul>\n<\/ul>\n<\/ul>\n<ul>\n<li aria-level=\"1\"><b>Nikon Eclipse Ti basic Fluorescence Microscope <\/b><b>(desplegable)<\/b><\/li>\n<\/ul>\n<ul>\n<ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Inverted Nikon Ti Eclipse fully motorized microscope<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Hamamatsu Orca R2 monochrome 1.3 MP CCD camera\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Objective lenses: 60x (1.4 NA), 40x, 10x and 4x<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Filter sets for DAPI, GFP, TRITC<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Configured for both NIS-Elements and the open-source Micro-manager suite for advanced long-term imaging experiments.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Differential interference contrast (DIC) for improved contrast in brightfield illumination.<\/span><\/li>\n<\/ul>\n<\/ul>\n<\/ul>\n<ul>\n<li aria-level=\"1\"><b>Nikon \/ EppendorfMicroinjection System <\/b><b>(desplegable)<\/b><\/li>\n<\/ul>\n<ul>\n<ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Inverted Nikon Ti Eclipse Microscope<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Eppendorfmicro-manipulation and injection system<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Colour fluorescence camera with filters for multi-color imaging\u00a0<\/span><\/li>\n<\/ul>\n<\/ul>\n<\/ul>\n<ul>\n<li aria-level=\"1\"><b>Nikon \/Hamilton ThornePhotoablation System <\/b><b>(desplegable)<\/b><\/li>\n<\/ul>\n<ul>\n<ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Inverted Nikon Ti Eclipse<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Hamilton Thorne XYClonephotoablation module<\/span><\/li>\n<\/ul>\n<\/ul>\n<\/ul>\n<ul>\n<li aria-level=\"1\"><b>Leica M165 Fluorescent Stereo-microscope <\/b><b>(desplegable)<\/b><\/li>\n<\/ul>\n<ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">High-end 1 x Plan Apo objective lens with 16.5:1 optical zoom\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Resolution of up to 1.1 microns<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Fluorescence (Hoechst\/DAPI, GFP\/FITC, and TRITC)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">8 MP Colour and 1.3 MP Monochromatic camera options<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"3\"><span style=\"font-weight: 400;\">Epi, Trans, and Oblique white light illumination options.<\/span><\/li>\n<\/ul>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_divider color=\u00bb#41a7d4&#8243; divider_style=\u00bbdotted\u00bb _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00bb1_2,1_2&#8243; use_custom_gutter=\u00bbon\u00bb gutter_width=\u00bb3&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb80px||80px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb|40px|||false|false\u00bb border_width_right=\u00bb1px\u00bb border_color_right=\u00bb#41a7d4&#8243; global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Applications<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Nanoscale imaging of fixed samples<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Dynamic imaging of cytoskeletal structures, focal adhesion formation, as well as endocytosis and vesicle dynamics in live cells.\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Single-molecule studies and localization microscopy modalities including N-STORM, Direct STORM, and PALM.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Fluorescent analysis of histological samples.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Microinjection of DNA, RNA, morpholinos, vital dyes, or pharmacological agents into cultured cells, oocytes, or embryos.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Non-contact ablation of targeted membranes or structures as a powerful tool for stem cell, cloning, and developmental biology areas.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">High-quality macro-to-micro imaging of both biological and non-biological samples<\/span><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb text_font=\u00bbPoppins||||||||\u00bb text_text_color=\u00bb#41a7d4&#8243; text_font_size=\u00bb20px\u00bb header_font=\u00bb|600|||||||\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>Services Offered<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb30px||||false|false\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<p>&#8211; Transmission electron microscopy of resin-embedded and non-embedded samples and elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX\/ EDS).<\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Super-resolution (SR) advice and consulting: we can help you evaluate and choose the best localization microscopy strategies for your samples.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">We can supply super-resolution specific reagents, including buffers and fluorochromes, and custom-labeled antibodies.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">STORM image processing: we can convert localization imaging data into SR images using both proprietary and open source software packages.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">TIRF imaging of membrane and\/or cytoskeletal dynamics in live adherent cells using commercial fluorescent labels such as DiR, FM4-64, and Cell-Light or user-supplied reagents.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Long-term (&gt;4 days) imaging of bacterial colonies and bacterial interactions using DIC, temperature control, autofocus, and multi-field imaging.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Routine multi-color fluorescent analysis of histological samples where confocal or other more advanced methods are not necessary.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Imaging of bone, muscle, or connective tissues in histological samples using circularly-polarised light.<\/span><\/li>\n<\/ul>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb background_color=\u00bb#235b78&#8243; custom_padding=\u00bb30px||30px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb4_4&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_text _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb header_3_font=\u00bbPoppins|700|||||||\u00bb header_3_text_color=\u00bb#FFFFFF\u00bb header_3_font_size=\u00bb34px\u00bb global_colors_info=\u00bb{}\u00bb]<\/p>\n<h3>Technical staff<\/h3>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00bb1&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_row column_structure=\u00bb1_2,1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb50px||50px||true|false\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb global_colors_info=\u00bb{}\u00bb][et_pb_blurb title=\u00bbMOLINA GIL, SARA\u00bb image=\u00bbhttps:\/\/ibima.eu\/wp-content\/uploads\/2024\/07\/Nano_SaraMolina_-320.jpg\u00bb image_icon_width=\u00bb40%\u00bb _builder_version=\u00bb4.27.0&#8243; _module_preset=\u00bbdefault\u00bb header_font=\u00bbPoppins|600|||||||\u00bb header_text_align=\u00bbcenter\u00bb body_text_align=\u00bbcenter\u00bb hover_enabled=\u00bb0&#8243; border_radii_image=\u00bbon|80px|80px|80px|80px\u00bb global_colors_info=\u00bb{}\u00bb title_text=\u00bbNano_SaraMolina_ 320&#8243; sticky_enabled=\u00bb0&#8243;]<\/p>\n<p>T\u00e9cnico de Microscop\u00eda<br \/>Microscopy Technician<\/p>\n<p>[\/et_pb_blurb][\/et_pb_column][et_pb_column type=\u00bb1_2&#8243; _builder_version=\u00bb4.20.4&#8243; _module_preset=\u00bbdefault\u00bb custom_padding=\u00bb||50px||false|false\u00bb global_colors_info=\u00bb{}\u00bb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Super-resolution and Conventional Fluorescence MicroscopyService DescriptionConventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional micros-copy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-12461","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Super-resolution and Conventional Fluorescence Microscopy - Ibima<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ibima.eu\/es\/super-resolution-and-conventional-fluorescence-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Super-resolution and Conventional Fluorescence Microscopy - Ibima\" \/>\n<meta property=\"og:description\" content=\"Super-resolution and Conventional Fluorescence MicroscopyService DescriptionConventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional micros-copy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera captures the whole field of view at the same time, [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ibima.eu\/es\/super-resolution-and-conventional-fluorescence-microscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"Ibima\" \/>\n<meta property=\"article:modified_time\" content=\"2024-07-25T15:28:47+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"7 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/ibima.eu\\\/es\\\/super-resolution-and-conventional-fluorescence-microscopy\\\/\",\"url\":\"https:\\\/\\\/ibima.eu\\\/es\\\/super-resolution-and-conventional-fluorescence-microscopy\\\/\",\"name\":\"Super-resolution and Conventional Fluorescence Microscopy - Ibima\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/ibima.eu\\\/es\\\/#website\"},\"datePublished\":\"2023-10-26T06:32:57+00:00\",\"dateModified\":\"2024-07-25T15:28:47+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/ibima.eu\\\/es\\\/super-resolution-and-conventional-fluorescence-microscopy\\\/#breadcrumb\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/ibima.eu\\\/es\\\/super-resolution-and-conventional-fluorescence-microscopy\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/ibima.eu\\\/es\\\/super-resolution-and-conventional-fluorescence-microscopy\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Portada\",\"item\":\"https:\\\/\\\/ibima.eu\\\/es\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Super-resolution and Conventional Fluorescence Microscopy\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/ibima.eu\\\/es\\\/#website\",\"url\":\"https:\\\/\\\/ibima.eu\\\/es\\\/\",\"name\":\"Ibima\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/ibima.eu\\\/es\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"es\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Super-resolution and Conventional Fluorescence Microscopy - Ibima","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/ibima.eu\/es\/super-resolution-and-conventional-fluorescence-microscopy\/","og_locale":"es_ES","og_type":"article","og_title":"Super-resolution and Conventional Fluorescence Microscopy - Ibima","og_description":"Super-resolution and Conventional Fluorescence MicroscopyService DescriptionConventional widefield fluorescence microscopy is still the best choice for many applications. The CCD and CMOS-based sensors used for conventional micros-copy are typically much more sensitive than the photomultiplier tubes used in confocal microscopes and flow cytometers. 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