Bruker Avance 600 MHz NMR spectrometer

Service Description

The NMR service is equipped with a High resolution NMR Spectrometers: a 600 MHz, spectrometer dedicated NMR studies that are more demanding in terms of sensitivity. This system is also equipped with a high resolution magic angle spinning (HR-MAS) NMR to analyze heterogeneous samples (tissue biopsies or partially insoluble compounds).
Applications of NMR spectroscopy to chemistry range from structure elucidation of small molecules to studies of ligand-protein interactions. In biomedicine, the use of NMR spectroscopy has expanded from the classical metabolic studies using enriched stable isotope tracers to the more recent NMR-based metabolomics of biofluids (urine, plasma, CSF).

Equipment

• Bruker Avance 600 MHz NMR spectrometer

– Ultra ShieldTM Plus 14.1T magnet

– 5 mm BBFOPLUS probe, optimized for X-nuclei direct observation in a frequency range between 31P and 15N

– 5 mm TXI probe, optimized for higher sensitivity 1H-nuclei observation

– TCI CryoProbe Prodigy, uses nitrogen-cooled RF coils and preamplifiers for 1H, 2H and 13C, delivering a 2.5x 1 H sensitivity boost

– HR-MAS Probe

Applications

– Metabolomic analysis of biofluids (urine, blood serum, etc.)

– Metabolomic analysis of intact tissue (biopsies)

Services Offered

• Experimental design advice and project plan consulting. Services Offered

• Acquisition of 1D NMR spectra at 600 MHz (1 H, 13C, 31P, etc.)

• Acquisition of basic 2D experiments: COSY, TOCSY, HSQC, HMBC, J-RES

– Nuclear Overhauser Effect experiments (NOE and ROE), Diffusion experiments (DOSY), selective excitation experiments.

• Acquisition of 1 H and 31P HR-MAS NMR spectra.

• Preparation of biofluid samples (urine, blood serum/plasma) for high resolution NMR spectroscopy following standardized protocols.

• Preparation of tissue samples for HR-MAS NMR spectroscopy.

• Basic processing of NMR spectra.