Histology

The equipment available at the unit together with the techniques continuously performed by the technical team allow for making qualitative and quantitative assessments of any tissue.

In particular, instruments for sectioning and polishing hard materials and histomorphometry equipment allow for the comprehensive analysis of bone tissue.
Through sectioning and polishing techniques, histological processing can be performed on samples that cannot be processed through conventional techniques, such as undecalcified bone tissue, samples that contain metallic implants...

Services

  • Procesamiento de muestras (Fijación, inclusión en parafina, corte y tinción) de muestras blandas mediante microtomo
  • Procesamiento de muestras (Fijación, crioprotección, inclusión en OCT y tinción) de muestras blandas mediante criostato
  • Procesamiento de muestras de alta dureza como hueso, implantes de distintas características (Titanio, acero inoxidable, hidroxiapatita, fosfato tricálcico, etc.), muestras de histología humana, muestras e implantes dentales, muestras metalográficas, etc.
  • Digitization of brightfield, darkfield, and fluorescent images in a single field or z-stack of up to 60 μm
  • Análisis histomorfométrico de muestras de hueso o músculo para medir cuantificar osteointegración, análisis de hueso cortical, histomorfometría humana, análisis de metástasis en hueso, etc.
  • Análisis de imágenes histológicas (Cuantificación de núcleos, análisis de fibras de colágeno, cuantificación de expresión de biomarcadores (IHC/IF), segmentación y clasificación de tejidos, cuantificación de angiogénesis, etc.)

Equipment

Conventional Histology Equipment

  • HM 360 THERMO SCIENTIFIC motorized rotary microtome with a blade holder accessory for methyl methacrylate samples
  • Criostato semiautomático LEICA CM 3050 S
  • Secuflow WALDNER fume cupboards (prepared for DAB developing)
    LEICA DM 1000 microscope with a LEICA ICC 50 HD camera and 4x, 10x, 20x, 4x, and 100x lenses (immersion)
  • OLYMPUS VS200 slide scanner for brightfield, darkfield, and polarized slides. 2x, 4x, 10x, 20x, and 40x lenses
  • LEICA APERIO VERSA 200 slide scanner with a loader for up to 200 slides for brightfield, fluorescent, and FISH slides. 1.25x, 4x, 10x, 20x, 40x, and 63x lenses (immersion). It includes nuclei quantification, membrane segmentation, and image analysis algorithms

Hard Tissue Equipment

  • EXAKT (401, 402, 510, 520, 530), equipment, EXAKT CL-CP 300 band saw system, and EXAKT 400 polishing system
  • BIOQUANT OSTEO histomorphometry equipment and software
Rates

Iván Durán Jiménez

Scientific Coordinator

ijduran@uma.es

Alejandro Domínguez Moreno

 Histology Technician

Contact: 952 36 76 27 | alejandro.dominguez@ibima.eu

Marta Carayol Gordillo

Electron Microscopy and Histology Technician

Contact: 952 36 76 37 |

mcarayol@ibima.eu