The equipment available at the unit together with the techniques continuously performed by the technical team allow for making qualitative and quantitative assessments of any tissue.

In particular, instruments for sectioning and polishing hard materials and histomorphometry equipment allow for the comprehensive analysis of bone tissue.
Through sectioning and polishing techniques, histological processing can be performed on samples that cannot be processed through conventional techniques, such as undecalcified bone tissue, samples that contain metallic implants...

• Sample processing (fixation, paraffin embedding, sectioning, and staining) of soft samples using a microtome.
• Sample processing (fixation, cryoprotection, OCT embedding, and staining) of soft samples using a cryostat.
• Processing of high-hardness samples such as bone, implants with various characteristics (titanium, stainless steel, hydroxyapatite, tricalcium phosphate, etc.), human histology samples, dental samples and implants, metallographic samples, etc.
• Digitization of brightfield, darkfield, and fluorescent images in a single field or z-stack of up to 60 μm
• Histomorphometric analysis of bone or muscle samples to measure and quantify osseointegration, cortical bone analysis, human histomorphometry, bone metastasis analysis, etc.
• Análisis de imágenes histológicas (Cuantificación de núcleos, análisis de fibras de colágeno, cuantificación de expresión de biomarcadores (IHC/IF), segmentación y clasificación de tejidos, cuantificación de angiogénesis, etc.)

Conventional Histology Equipment

• HM 360 THERMO SCIENTIFIC motorized rotary microtome with a blade holder accessory for methyl methacrylate samples
• Criostato semiautomático LEICA CM 3050 S
• Secuflow WALDNER fume cupboards (prepared for DAB developing)
  LEICA DM 1000 microscope with a LEICA ICC 50 HD camera and 4x, 10x, 20x, 4x, and 100x lenses (immersion)
• OLYMPUS VS200 slide scanner for brightfield, darkfield, and polarized slides. 2x, 4x, 10x, 20x, and 40x lenses
• LEICA APERIO VERSA 200 slide scanner with a loader for up to 200 slides for brightfield, fluorescent, and FISH slides. 1.25x, 4x, 10x, 20x, 40x, and 63x lenses (immersion). It includes nuclei quantification, membrane segmentation, and image analysis algorithms

Hard Tissue Equipment

• EXAKT (401, 402, 510, 520, 530), equipment, EXAKT CL-CP 300 band saw system, and EXAKT 400 polishing system
• BIOQUANT OSTEO histomorphometry equipment and software