Non-alcoholic fatty liver disease (NAFLD) is a multifactorial chronic inflammatory disease that is prevalent in 1 of 4 individuals with a significant personal, socioeconomic and healthcare burden, especially at the later, more severe inflammatory stage of disease – non-alcoholic steatohepatitis (NASH). Despite the severe negative impact of the disease on society, NAFLD remains difficult to diagnose and treat. Additionally, the molecular mechanisms underlying the transition from health to fatty liver to NASH remain poorly understood due to the lack of models that faithfully reflect the complexity of human disease. Hence, Halt-RONIN aims to uncover the early triggers of disease initiation and complex mechanistic drivers of disease progression by implementing a systems biology approach with integrative disease modelling resulting in opportunities for the improvement of the existing detection methods, providing a blueprint to inform personalized intervention strategies and drug discovery for NAFLD. To achieve this goal, Halt-RONIN will combine experimental data from advanced in vitro and in vivo models with multimodal data from extensive human NAFLD cohorts and biobanks and use in silico machine learning approaches, to discover new biomarkers and molecular targets specific to each stage of the health-to-disease transition. By validating preclinical experimental findings with real-world data, RONIN will allow for the discovery of novel biomarkers and molecular targets that are specific to the individual patient’s pathology. Consequently, healthcare professionals will gain the tools and knowledge required to diagnose and establish guidelines for the prevention and treatment of inflammation-driven health to disease. As such, in the long-term RONIN will decrease the number of NAFL patients who progress into NASH and provide disease-modifying strategies to improve patient outcomes.

• • • UNIVERSIDAD COMPLUTENSE DE MADRID – UCM
    • BIOPREDIC INTERNATIONAL SARL – Biopredic International
    • ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOCIENCIAS – CIC BIOGUNE
    • BILKENT UNIVERSITESI VAKIF – BILKENTUNIVERSITY BILIM KENTI
    • SERVICIO ANDALUZ DE SALUD (SAS)
    • IZMIR BIYOTIP VE GENOM MERKEZI – IZMIR BIOMEDICINE AND GENOME CENTER
    • INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
    • UNIVERSIDADE NOVA DE LISBOA – UNL
    • FACULDADE DE FARMACIA DA UNIVERSIDADE DE LISBOA – FFUL
    • UNIVERSIDAD DE MALAGA – UMA
    • ANAXOMICS BIOTECH SL – AX
    • SERVICIO MADRILENO DE SALUD – SERMAS
    • FUNDACIO CLINIC PER A LA RECERCA BIOMEDICA – FCRB-CERCA
    • FUNDACION INSTITUTO DE INVESTIGACION MARQUES DE VALDECILLA – IDIVAL
    • AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS – CSIC
    • UNIVERSITY OF GLASGOW (external partner)
    • THE UNIVERSITY OF EDINBURGH – UEDIN (external partner)