Flow Cytometry
Description
Flow cytometry is a highly effective method for analyzing thousands of cells or other particles in mere minutes. Flow cytometers detect the fluorescence, size, and complexity of particles on which beams of visible and fluorescent light are shined. Using this method, statistically valid data can quickly be generated by counting a large number of particles, which would be extremely difficult using traditional microscopy.
Flow cytometers are very well established in hospitals for routine diagnostics and are now widely used for different types of research applications, from marine biology to stem cell research
Services
- Technical advising and support
- Design of multicolor panels and cytometry experiments
- Advising on sample preparation
- Analytical cytometry and spectral cell sorting services
- Data processing and analysis
- Training in the use of analytical equipment
- Cell cycle analysis
- Cell viability, mitochondrial energy balance, apoptosis, and cytotoxicity analysis (Annexin V, JC1, PI)
- Detection of fluorescent protein expression (GFP, YFP, CFP)
- Cellular proliferation detection with CFSE
- Detection of naturally fluorescent micro-organisms (Marine Biology)
Equipment
Beckman Coulter GALLIOS
Analytical cytometer equipped with a 3-laser system (solid state diode): blue (488 nm), red (638 nm), and violet (405 mm)
Beckman Coulter CytoFLEX
Analytical cytometer equipped with a 4-laser system: blue (488 nm), yellow/green (561nm), red (638 nm), and violet (405 mm) and APD detectors. It has a 96-well plate adapter
Becton Dickinson BD FACSCalibur ( Hospital Civil Laboratory)
Analytical cytometer equipped with a 2-laser system (solid state diode): blue (488 nm) and red (638 nm)
Becton Dickinson BD FACSCantoII (Hospital Civil Laboratory)
Analytical cytometer equipped with a 2-laser system (solid state diode): blue (488 nm) and red (638 nm)
CYTEK AURORA CS
Cell sorter spectral cytometer equipped with a 3-laser solid state system: blue (488 nm), red (638 nm), and violet (405 mm) and APD detectors.
The wide range of detectors allows for detecting any fluorochrome excited by the lasers
Beckman Coulter Astrios MoFlo Sorter ( Hospital Civil Laboratory)
Analytical cytometer equipped with a 4-laser system: blue(488 nm), red (640 nm), violet (405 mm), and yellow (561nm)
Rates
Contact
Elena González Muñoz
Scientific Coordinator
