Cell Cultures
Description
IBIMA plataforma BIONAND offers a cell culture unit to research groups at the Institute and groups from other public or private institutions. The unit includes two separate spaces dedicated to managing established cell lines and primary cultures (mainly murine and human cultures), respectively, in sterile conditions, categorized as category 1 and 2 biological risk (containment level 2) and a room for cell cultures with viral vectors (containment level 2). In addition, to avoid the contamination of samples with pathogens, the unit also has a quarantine room dedicated to temporarily housing any new cell entering the facility until any possible pathogens are detected and treated or ruled out
Services
• Maintenance and disinfection of biosafety hoods
• Maintenance and disinfection of incubators
• Regular changing of CO2 incubator water
• Maintenance and disinfection of thermostatic baths
• Periodic analysis of mycoplasma contamination in incubators
• Removal of solid waste and treatment and removal of liquid waste
• Equipment and facilities for the use of viral vectors in cell cultures
• Daily temperature and CO2 control in incubators
• Annual certification of cabinets and incubators
Specific
• Advising on the acquisition and maintenance of cell lines
• Cryopreservation of samples
• Analysis of mycoplasma contamination
• Viability testing
Equipment
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Class II A2 biosafety hoods
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Nucleofector 4D electroporation nucleofector system (AMAXA-Lonza)
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Refrigerated centrifuges
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Inverted brightfield microscope with phase and fluorescence contrast with camera
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CO2 incubators
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Cryopreservation room with three liquid nitrogen tanks
Rates
Contact
Elena González Muñoz
Scientific Coordinator
Concepción Oca Gil
Animal Facility and Cell Culture Technician